Genotypic identification and characterization of species and strains within the genus Candida by using random amplified polymorphic DNA.

@article{Lehmann1992GenotypicIA,
  title={Genotypic identification and characterization of species and strains within the genus Candida by using random amplified polymorphic DNA.},
  author={Paul F. Lehmann and David Lin and Brent A. Lasker},
  journal={Journal of clinical microbiology},
  year={1992},
  volume={30 12},
  pages={3249-54}
}
Random amplified polymorphic DNA (RAPD) was used to better characterize the genotypic relatedness among medically important Candida species. By using short oligomer primers (10-mers) with arbitrarily chosen sequences in the polymerase chain reaction, distinctive and reproducible sets of polymerase chain reaction products were observed for isolates of C. albicans, C. lusitaniae, C. tropicalis, and Torulopsis (Candida) glabrata. The RAPD analysis differentiated a physiologically homogeneous panel… CONTINUE READING