Genotypic analyses of Vibrio parahaemolyticus and development of a pandemic group-specific multiplex PCR assay.

@article{Okura2003GenotypicAO,
  title={Genotypic analyses of Vibrio parahaemolyticus and development of a pandemic group-specific multiplex PCR assay.},
  author={Masatoshi Okura and Ro Osawa and Atsushi Iguchi and Eiji Arakawa and Jun Terajima and Haruo Watanabe},
  journal={Journal of clinical microbiology},
  year={2003},
  volume={41 10},
  pages={
          4676-82
        }
}
A total of 54 Vibrio parahaemolyticus strains including pandemic O3:K6 strains and newly emerged O4:K68, O1:K25, O1:K26, and O1:K untypeable strains (collectively referred to as the "pandemic group") were examined for their pulsed-field gel electrophoresis (PFGE) and arbitrarily primed PCR (AP-PCR) profiles and for the presence or absence of genetic marker DNA sequences, toxRS/new or orf8, that had been reported elsewhere to be specific for the pandemic group. Both PFGE and AP-PCR analyses… CONTINUE READING
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