Genotype-specific influence on nitric oxide synthase gene expression, protein concentrations, and enzyme activity in cultured human endothelial cells.

@article{Song2003GenotypespecificIO,
  title={Genotype-specific influence on nitric oxide synthase gene expression, protein concentrations, and enzyme activity in cultured human endothelial cells.},
  author={Junghan Song and Yeomin Yoon and Kyoung Un Park and Junwan Park and Young Joon Hong and Seung Ho Hong and Jin Q. Kim},
  journal={Clinical chemistry},
  year={2003},
  volume={49 6 Pt 1},
  pages={847-52}
}
BACKGROUND The results of studies on the association of ecNOS polymorphisms and vascular diseases are inconsistent. To explore the nature of this interaction in the absence of confounding factors, such as smoking, we measured ecNOS mRNA, protein, and enzyme activity in cultured human umbilical vein endothelial cells (HUVECs) with and without ecNOS polymorphisms. METHODS We identified a T(-786)-->C polymorphism in the promoter region, the intron 4 variable number of tandem repeats (VNTR), the… CONTINUE READING