Genomics accelerated isolation of a new stem rust avirulence gene-wheat resistance gene pair.

@article{Upadhyaya2021GenomicsAI,
  title={Genomics accelerated isolation of a new stem rust avirulence gene-wheat resistance gene pair.},
  author={Narayana M. Upadhyaya and Rohit Mago and Vinay Panwar and Tim Hewitt and Mingcheng Luo and Jian Chen and Jana Sperschneider and Hoa Nguyen-Phuc and Aihua Wang and Diana Ortiz and Luch Hac and Dhara R Bhatt and Feng Li and Jianping Zhang and Michael Ayliffe and Melania Figueroa and Kostya Kanyuka and Jeffrey G. Ellis and Peter N. Dodds},
  journal={Nature plants},
  year={2021}
}
Stem rust caused by the fungus Puccinia graminis f. sp. tritici (Pgt) is a devastating disease of the global staple crop wheat. Although this disease was largely controlled in the latter half of the twentieth century, new virulent strains of Pgt, such as Ug99, have recently evolved1,2. These strains have caused notable losses worldwide and their continued spread threatens global wheat production. Breeding for disease resistance provides the most cost-effective control of wheat rust diseases3. A… 
The stem rust effector protein AvrSr50 escapes Sr50 recognition by a substitution in a single surface exposed residue.
TLDR
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The Pca203 reference marks the third fully-phased chromosome-level assembly of a cereal rust to date, and it is demonstrated that the chromosomes of these three Puccinia species are syntenous and that chromosomal size variations are primarily due to differences in repeat element content.
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It is shown that signal peptide prediction is essential for accurate effector prediction, as EffectorP 3.0 recognizes a cytoplasmic signal also in intracellular, non-secreted proteins.
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TLDR
Comparison transcriptome-based and orthology-guided approaches are used to characterize gene expression changes associated with Pgt infection in susceptible and resistant Triticum aestivum genotypes as well as the non-host Brachypodium distachyon and report several processes potentially linked to susceptibility to Pgt.

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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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