Genomic tagging reveals a random association of endogenous PtdIns5P 4-kinases IIα and IIβ and a partial nuclear localization of the IIα isoform

@inproceedings{Wang2010GenomicTR,
  title={Genomic tagging reveals a random association of endogenous PtdIns5P 4-kinases IIα and IIβ and a partial nuclear localization of the IIα isoform},
  author={Minchuan Wang and Nicholas J. Bond and Andrew J. Letcher and Jonathan P. Richardson and Kathryn S. Lilley and Robin F. Irvine and Jonathan H Clarke},
  booktitle={The Biochemical journal},
  year={2010}
}
PtdIns5P 4-kinases IIalpha and IIbeta are cytosolic and nuclear respectively when transfected into cells, including DT40 cells [Richardson, Wang, Clarke, Patel and Irvine (2007) Cell. Signalling 19, 1309-1314]. In the present study we have genomically tagged both type II PtdIns5P 4-kinase isoforms in DT40 cells. Immunoprecipitation of either isoform from tagged cells, followed by MS, revealed that they are associated directly with each other, probably by heterodimerization. We quantified the… CONTINUE READING

Figures, Tables, and Topics from this paper.

Citations

Publications citing this paper.
SHOWING 1-10 OF 26 CITATIONS

Investigating the potential role of PIP4Ks in PI3K/Akt signalling

VIEW 11 EXCERPTS
CITES BACKGROUND
HIGHLY INFLUENCED

Nuclear phosphoinositides and their roles in cell biology and disease.

VIEW 5 EXCERPTS
CITES BACKGROUND & RESULTS
HIGHLY INFLUENCED

Phosphatidylinositol Kinases and Phosphatases in Entamoeba histolytica

VIEW 7 EXCERPTS
CITES BACKGROUND
HIGHLY INFLUENCED

Exploring phosphatidylinositol 5-phosphate 4-kinase function

VIEW 8 EXCERPTS
CITES BACKGROUND & RESULTS

Phosphoinositides: tiny lipids with giant impact on cell regulation.

VIEW 4 EXCERPTS
CITES BACKGROUND
HIGHLY INFLUENCED

Role of phosphatidylinositol 5-phosphate 4-kinase α in zebrafish development.

VIEW 4 EXCERPTS
CITES BACKGROUND
HIGHLY INFLUENCED

Phosphoinositide signalling in the nucleus.

VIEW 8 EXCERPTS
CITES BACKGROUND
HIGHLY INFLUENCED

References

Publications referenced by this paper.
SHOWING 1-10 OF 42 REFERENCES

A perinuclear actin cap regulates nuclear shape.