Genomic segments cloning and analysis of Cotesia plutellae polydnavirus using plasmid capture system.

@article{Choi2005GenomicSC,
  title={Genomic segments cloning and analysis of Cotesia plutellae polydnavirus using plasmid capture system.},
  author={J. Choi and J. Roh and J. Kang and H. Shim and S. Woo and B. Jin and M. S. Li and Y. Je},
  journal={Biochemical and biophysical research communications},
  year={2005},
  volume={332 2},
  pages={
          487-93
        }
}
  • J. Choi, J. Roh, +5 authors Y. Je
  • Published 2005
  • Biology, Medicine
  • Biochemical and biophysical research communications
Cotesia plutellae polydnaviruses (CpBV) has a segmented genome consisting of multiple circular double stranded DNAs. Recently, we have developed an easy, simple, and convenient system based on Tn7 transposition in order to clone genomic segments of CpBV in Escherichia coli cell and designated plasmid capture system (PCS). The PCS donor-S transferred a pUC19 origin of replication and an ampicillin resistance marker into CpBV genomic DNA by in vitro transposition. Through PCS system, we were able… Expand
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