Genomic organization and physical mapping of the transfer RNA genes in Escherichia coli K12.

@article{Komine1990GenomicOA,
  title={Genomic organization and physical mapping of the transfer RNA genes in Escherichia coli K12.},
  author={Yasuomi Komine and Taiji Adachi and Hachiro Inokuchi and Haruo Ozeki},
  journal={Journal of molecular biology},
  year={1990},
  volume={212 4},
  pages={579-98}
}
By using a set of 476 ordered DNA clones (in lambda phage vector) that covers the entire chromosome of Escherichia coli K12, we have made an exhaustive survey of tRNA genes in the E. coli genome. Ultraviolet-irradiated bacteria were separately infected with each of the 476 clones and the RNA molecules produced upon infection were labeled with 32P. The labeled tRNAs were separated by gel electrophoresis and then characterized by fingerprinting analysis. Fifty-nine of the 476 clones produced… CONTINUE READING
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