Genomic insights that advance the species definition for prokaryotes.

@article{Konstantinidis2005GenomicIT,
  title={Genomic insights that advance the species definition for prokaryotes.},
  author={Konstantinos T. Konstantinidis and James M. Tiedje},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2005},
  volume={102 7},
  pages={
          2567-72
        }
}
  • K. Konstantinidis, J. Tiedje
  • Published 15 February 2005
  • Biology
  • Proceedings of the National Academy of Sciences of the United States of America
To help advance the species definition for prokaryotes, we have compared the gene content of 70 closely related and fully sequenced bacterial genomes to identify whether species boundaries exist, and to determine the role of the organism's ecology on its shared gene content. We found the average nucleotide identity (ANI) of the shared genes between two strains to be a robust means to compare genetic relatedness among strains, and that ANI values of approximately 94% corresponded to the… 

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