Genomic clones of the human liver-type phosphofructokinase.


Genomic clones of human liver phosphofructokinase (PFK) were isolated by screening a gene bank enriched for chromosome 21 sequences with two synthetic oligonucleotide probes designed from peptide sequences of purified human liver PFK. A 3.3 Kb fragment derived from the genomic clones was sub-cloned and designated to pG-PFKL 3.3. It hybridized with a 3.5 Kb mRNA on Northern blots and was able to enrich selectively for liver PFK mRNA by hybrid-selection. These results demonstrated that the isolated clones contain sequences homologous to human PFKL mRNA. When hybridized to genomic DNA blots pG-PFKL 3.3 reacted with the same 3.3 Kb BamHI fragment in both human DNA and DNA of the mouse/human hybrid line WA17 which contains human chromosome 21 as the only human chromosome. These data confirm the assignment of the PFKL gene to chromosome 21.

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@article{Levanon1986GenomicCO, title={Genomic clones of the human liver-type phosphofructokinase.}, author={Ditsa Levanon and E. Danciger and Naomi Dafni and Yoram Groner}, journal={Biochemical and biophysical research communications}, year={1986}, volume={141 1}, pages={374-80} }