Genomic analysis of single cytokeratin-positive cells from bone marrow reveals early mutational events in breast cancer.

@article{Schardt2005GenomicAO,
  title={Genomic analysis of single cytokeratin-positive cells from bone marrow reveals early mutational events in breast cancer.},
  author={Julian A Schardt and Manfred Meyer and Claudia H. Hartmann and Falk Schubert and Oleg Schmidt-Kittler and Christine Fuhrmann and Bernhard Polzer and Marco Petronio and Roland Eils and Christoph A Klein},
  journal={Cancer cell},
  year={2005},
  volume={8 3},
  pages={227-39}
}
Chromosomal instability in human breast cancer is known to take place before mammary neoplasias display morphological signs of invasion. We describe here the unexpected finding of a tumor cell population with normal karyotypes isolated from bone marrow of breast cancer patients. By analyzing the same single cells for chromosomal aberrations, subchromosomal allelic losses, and gene amplifications, we confirmed their malignant origin and delineated the sequence of genomic events during breast… CONTINUE READING