Genomic Organization and Expression Pattern of Mouse Neuroglycan C in the Cerebellar Development*

@article{Aono2000GenomicOA,
  title={Genomic Organization and Expression Pattern of Mouse Neuroglycan C in the Cerebellar Development*},
  author={S. Aono and Hiroomi Keino and Takao Ono and Yoko Yasuda and Yoshihito Tokita and Fumiko Matsui and Masatoshi Taniguchi and Shin‐ichi Sonta and Atsuhiko Oohira},
  journal={The Journal of Biological Chemistry},
  year={2000},
  volume={275},
  pages={337 - 342}
}
Neuroglycan C (NGC) is a membrane-spanning chondroitin sulfate proteoglycan with an epidermal growth factor module that is expressed predominantly in the brain. Cloning studies with mouse NGC cDNA revealed the expression of three distinct isoforms (NGC-I, -II, and -III) in the brain and revealed that the major isoform showed 94.3% homology with the rat counterpart. The NGC gene comprised six exons, was approximately 17 kilobases in size, and was assigned to mouse chromosome band 9F1 by… 

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Expression and identification of a new splice variant of neuroglycan C, a transmembrane chondroitin sulfate proteoglycan, in the human brain
TLDR
The expression of NGC in the human brain, mainly in the hippocampus, was studied and some observations were confirmed by conducting experiments using rat brain, and NGC‐IV, which was first found in the present study, had the shortest cytoplasmic domain among the four variants.
Glycosylation Site for Chondroitin Sulfate on the Neural Part-time Proteoglycan, Neuroglycan C*
TLDR
The CS glycosylation was not necessary for intracellular trafficking of NGC to the cell surface at least in Neuro 2a cells, suggesting that the addition of CS chains to the NGC core protein is regulated in a manner different from that of other CS proteoglycans.
Phosphorylation of Neuroglycan C, a Brain-specific Transmembrane Chondroitin Sulfate Proteoglycan, and Its Localization in the Lipid Rafts*
TLDR
This is the first report to demonstrate that NGC can be phosphorylated both intracellularly and pericellularly, and the findings suggest that a kinase with a specificity similar to that of casein kinase II is responsible for the NGC ectodomain phosphorylation.
Molecular interactions of neural chondroitin sulfate proteoglycans in the brain development.
TLDR
This review mainly summarizes recent studies on the involvement of these three classes of proteoglycan in cell-cell and cell-substratum interactions during the brain development.
Proteoglycans in brain development
TLDR
A function for aggrecan is suggested in the specification of a sub-set of glia precursors that might give rise to astrocytes in vivo, as part of the extracellular or cell-surface milieu of most tissues and organ systems.
Differential neuroglycan C expression during retinal degeneration in Rpe65−/− mice
TLDR
During retinal degeneration in Rpe65−/− mice, NGC expression is induced in the neural retina, but not in the RPE, where NGC is expressed at highest levels.
Neuroglycan C Is a Novel Midkine Receptor Involved in Process Elongation of Oligodendroglial Precursor-like Cells*
TLDR
It is concluded that neuroglycan C is a novel component of midkine receptors involved in process elongation in undifferentiated bipolar CG-4 cells.
Chondroitin sulfate proteoglycans in the brain.
Identification of Neurite Outgrowth-promoting Domains of Neuroglycan C, a Brain-specific Chondroitin Sulfate Proteoglycan, and Involvement of Phosphatidylinositol 3-Kinase and Protein Kinase C Signaling Pathways in Neuritogenesis*
TLDR
It is reported that the recombinant ectodomain of NGC core protein enhances neurite outgrowth from rat neocortical neurons in culture, and both protein kinase C (PKC) inhibitors and phosphatidylinositol 3-kinase (PI3K) inhibitors attenuated the NGC-mediated neurites outgrowth in a dose-dependent manner, suggesting that NGC promotes neuriteOutgrowth via PI3K and PKC pathways.
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