Genomic Nucleosome Organization Reconstituted with Pure Proteins

@article{Krietenstein2016GenomicNO,
  title={Genomic Nucleosome Organization Reconstituted with Pure Proteins},
  author={Nils Krietenstein and Megha Wal and Shinya Watanabe and Bongsoo Park and Craig L. Peterson and B. Franklin Pugh and Philipp Korber},
  journal={Cell},
  year={2016},
  volume={167},
  pages={709-721.e12}
}
Chromatin remodelers regulate genes by organizing nucleosomes around promoters, but their individual contributions are obfuscated by the complex in vivo milieu of factor redundancy and indirect effects. Genome-wide reconstitution of promoter nucleosome organization with purified proteins resolves this problem and is therefore a critical goal. Here, we reconstitute four stages of nucleosome architecture using purified components: yeast genomic DNA, histones, sequence-specific Abf1/Reb1, and… CONTINUE READING
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