Genome-wide screen for inositol auxotrophy in Saccharomyces cerevisiae implicates lipid metabolism in stress response signaling

@article{VillaGarca2010GenomewideSF,
  title={Genome-wide screen for inositol auxotrophy in Saccharomyces cerevisiae implicates lipid metabolism in stress response signaling},
  author={Manuel J. Villa-Garc{\'i}a and Myung Sun Choi and Flora I. Hinz and Mar{\'i}a L. Gaspar and Stephen A. Jesch and Susan A Henry},
  journal={Molecular Genetics and Genomics},
  year={2010},
  volume={285},
  pages={125-149}
}
Inositol auxotrophy (Ino− phenotype) in budding yeast has classically been associated with misregulation of INO1 and other genes involved in lipid metabolism. To identify all non-essential yeast genes that are necessary for growth in the absence of inositol, we carried out a genome-wide phenotypic screening for deletion mutants exhibiting Ino− phenotypes under one or more growth conditions. We report the identification of 419 genes, including 385 genes not previously reported, which exhibit… CONTINUE READING