Genome-wide nuclear morphology screen identifies novel genes involved in nuclear architecture and gene-silencing in Saccharomyces cerevisiae.

@article{Teixeira2002GenomewideNM,
  title={Genome-wide nuclear morphology screen identifies novel genes involved in nuclear architecture and gene-silencing in Saccharomyces cerevisiae.},
  author={Maria Teresa Bustamente Teixeira and Bernard Dujon and Emmanuelle Fabre},
  journal={Journal of molecular biology},
  year={2002},
  volume={321 4},
  pages={551-61}
}
Organisation of the cell nucleus is crucial for the regulation of gene expression but little is known about how nuclei are structured. To address this issue, we designed a genomic screen to identify factors involved in nuclear architecture in Saccharomyces cerevisiae. This screen is based on microscopic monitoring of nuclear pore complexes and nucleolar proteins fused with the green fluorescent protein in a collection of approximately 400 individual deletion mutants. Among the 12 genes… CONTINUE READING