Genome-wide mutant collections: toolboxes for functional genomics.

  title={Genome-wide mutant collections: toolboxes for functional genomics.},
  author={Paulo S. R. Coelho and A Naresh Kumar and Michael Snyder},
  journal={Current opinion in microbiology},
  volume={3 3},
The sequencing of entire genomes has led to the identification of many genes. A future challenge will be to determine the function of all of the genes of an organism. One of the best ways to ascertain function is to disrupt genes and determine the phenotype of the resulting organism. Novel large-scale approaches for generating gene disruptions and analyzing the resulting phenotype are underway in the budding yeast Saccharomyces cerevisiae and other organisms including flies, Mycoplasma, worms… CONTINUE READING