Genome-wide mapping of promoter-anchored interactions with close to single-enhancer resolution

  title={Genome-wide mapping of promoter-anchored interactions with close to single-enhancer resolution},
  author={Pelin Sahl{\'e}n and I. Abdullayev and Daniel Ramsk{\"o}ld and L. Matskova and Nemanja Rilakovic and Britta L{\"o}tstedt and T. Albert and J. Lundeberg and R. Sandberg},
  journal={Genome Biology},
  • Pelin Sahlén, I. Abdullayev, +6 authors R. Sandberg
  • Published 2015
  • Biology, Medicine
  • Genome Biology
  • Although the locations of promoters and enhancers have been identified in several cell types, we still have limited information on their connectivity. We developed HiCap, which combines a 4-cutter restriction enzyme Hi-C with sequence capture of promoter regions. Applying the method to mouse embryonic stem cells, we identified promoter-anchored interactions involving 15,905 promoters and 71,984 distal regions. The distal regions were enriched for enhancer marks and transcription, and had a mean… CONTINUE READING
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