Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering

@article{Garst2017GenomewideMO,
  title={Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering},
  author={Andrew D. Garst and Marcelo C Bassalo and Gur Pines and Sean A. Lynch and Andrea L. Halweg-Edwards and Rongming Liu and Liya Liang and Zhiwen Wang and Ramsey I Zeitoun and William G Alexander and Ryan T. Gill},
  journal={Nature Biotechnology},
  year={2017},
  volume={35},
  pages={48-55}
}
Improvements in DNA synthesis and sequencing have underpinned comprehensive assessment of gene function in bacteria and eukaryotes. Genome-wide analyses require high-throughput methods to generate mutations and analyze their phenotypes, but approaches to date have been unable to efficiently link the effects of mutations in coding regions or promoter elements in a highly parallel fashion. We report that CRISPR–Cas9 gene editing in combination with massively parallel oligomer synthesis can enable… CONTINUE READING
Recent Discussions
This paper has been referenced on Twitter 114 times over the past 90 days. VIEW TWEETS

Citations

Publications citing this paper.
Showing 1-10 of 38 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 88 references

A homology integrated CRISPR-Cas (HI-CRISPR) system for one-step multi-gene disruptions in Saccharomyces cerevisiae

  • Z Bao
  • ACS Synth. Biol
  • 2015

Metabolic engineering of Escherichia coli using CRISPR-Cas9 meditated genome

  • Y Li
  • editing. Metab. Eng. 31,
  • 2015

Similar Papers

Loading similar papers…