Genome-wide mapping of foamy virus vector integrations into a human cell line.

@article{Nowrouzi2006GenomewideMO,
  title={Genome-wide mapping of foamy virus vector integrations into a human cell line.},
  author={Ali Nowrouzi and Marcus Dittrich and Chuck Klanke and Martin Heinkelein and Matthias Rammling and Thomas Dandekar and Christof von Kalle and Axel Rethwilm},
  journal={The Journal of general virology},
  year={2006},
  volume={87 Pt 5},
  pages={1339-47}
}
Integration-site selection by retroviruses and retroviral vectors has gained increased scientific interest. Foamy viruses (FVs) constitute a unique subfamily (Spumavirinae) of the family Retroviridae, for which the integration pattern into the human genome has not yet been determined. To accomplish this, 293 cells were transduced with FV vectors and the integration sites into the cellular genome were determined by a high-throughput method based on inverse PCR. For comparison, a limited number… CONTINUE READING