Genome-wide lethality screen identifies new PI4,5P2 effectors that regulate the actin cytoskeleton.

@article{Audhya2004GenomewideLS,
  title={Genome-wide lethality screen identifies new PI4,5P2 effectors that regulate the actin cytoskeleton.},
  author={Anjon Audhya and Robbie Loewith and Ainslie B. Parsons and Lu Gao and Mitsuaki Tabuchi and Huilin Zhou and Charles Boone and Michael N. Hall and Scott D Emr},
  journal={The EMBO journal},
  year={2004},
  volume={23 19},
  pages={3747-57}
}
To further understand the roles played by the essential phosphoinositide PI4,5P(2), we have used a synthetic lethal analysis, which systematically combined the mss4(ts) mutation, partially defective in PI4P 5-kinase activity, with each of approximately 4700 deletion mutations. This genomic screening technique uncovered numerous new candidate effectors and regulators of PI4,5P(2) in yeast. In particular, we identified Slm1 (Yil105c), a previously uncharacterized PI4,5P(2) binding protein. Like… CONTINUE READING

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