Genome-wide identification of intron fragment insertion mutations and their potential use as SCAR molecular markers in the soybean

@article{Shu2010GenomewideIO,
  title={Genome-wide identification of intron fragment insertion mutations and their potential use as SCAR molecular markers in the soybean},
  author={Yongjun Shu and Yong Li and Yanming Zhu and Zhenlei Zhu and Dekang Lv and Xi Bai and Hua Cai and Wei Ji and Dianjing Guo},
  journal={Theoretical and Applied Genetics},
  year={2010},
  volume={121},
  pages={1-8}
}
Introns often have a high probability of mutation as a result of DNA insertions and deletions (indels). In this study, 503 introns with exon-derived insertions were identified using a comprehensive search of the soybean genome. Of the 375 pairs of PCR primer sets designed for the loci in question, 161 primer sets amplified length polymorphism among nine soybean varieties and were identified as soybean gene-intron-driven functional sequence characterized amplified region (SCAR) markers. These… CONTINUE READING