Genome-wide identification of Isw2 chromatin-remodeling targets by localization of a catalytically inactive mutant.

@article{Gelbart2005GenomewideIO,
  title={Genome-wide identification of Isw2 chromatin-remodeling targets by localization of a catalytically inactive mutant.},
  author={Marnie E Gelbart and Nurjana Bachman and Jeffrey J. Delrow and Jef D. Boeke and Toshio Tsukiyama},
  journal={Genes & development},
  year={2005},
  volume={19 8},
  pages={942-54}
}
Isw2 ATP-dependent chromatin-remodeling activity is targeted to early meiotic and MATa-specific gene promoters in Saccharomyces cerevisiae. Unexpectedly, preferential cross-linking of wild-type Isw2p was not detected at these loci. Instead, the catalytically inactive Isw2p-K215R mutant is enriched at Isw2 targets, suggesting that Isw2p-K215R, but not wild-type Isw2p, is a sensitive chromatin immunoprecipitation (ChIP) reagent for marking sites of Isw2 activity in vivo. Genome-wide ChIP analyses… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 48 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 66 references

In vivo chromatin remodeling by yeast ISWI homologs Isw 1 p and Isw 2 p

  • J. M. Kim, S. Vanguri, J. D. Boeke, A. Gabriel, D. F. Voytas
  • Genes & Dev .
  • 1998
Highly Influential
3 Excerpts

Integration of the yeast ret - rotransposon Ty 1 is targeted to regions upstream of genes transcribed by RNA polymerase III

  • A. E. Ehrenhofer-Murray
  • Genes & Dev .
  • 2004
1 Excerpt

Similar Papers

Loading similar papers…