Genome-wide identification of Ago2 binding sites from mouse embryonic stem cells with and without mature microRNAs

Abstract

MicroRNAs (miRNAs) are 19–22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP… (More)
DOI: 10.1038/nsmb0911-1084a

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