Genome-wide identification and analysis of transcribed enhancers during macrophage polarization

Abstract

Background Macrophages are sentinel cells that play essential role in tissue homeostasis and host defence. Owing to their plasticity, macrophages acquire a range of functional phenotypes in response to microenvironmental stimuli, of which M1 (IFNγ) and M2 (IL-4/IL-13) phenotypes are well-known for their opposing proand antiinflammatory roles. Enhancers have emerged as regulatory DNA elements crucial for transcriptional activation of gene expression, with recent studies highlighting their importance in macrophages. Results Using cap analysis of gene expression (CAGE) and epigenetic data, we identify on a large-scale transcribed enhancers in mouse macrophages, their time kinetics and target protein-coding genes. We observe an increase in target gene expression, concomitant with increasing numbers of associated enhancers and find that genes associated to many enhancers show a shift towards stronger enrichment for macrophage-specific biological not peer-reviewed) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was . http://dx.doi.org/10.1101/163519 doi: bioRxiv preprint first posted online Jul. 14, 2017;

7 Figures and Tables

Cite this paper

@inproceedings{Denisenko2017GenomewideIA, title={Genome-wide identification and analysis of transcribed enhancers during macrophage polarization}, author={Elena Denisenko and Reto Guler and Musa M. Mhlanga and Harukazu Suzuki and Frank Brombacher and Sebastian Schmeier}, year={2017} }