Genome-wide functional annotation of dual-specificity protein- and lipid-binding modules that regulate protein interactions.

@article{Chen2012GenomewideFA,
  title={Genome-wide functional annotation of dual-specificity protein- and lipid-binding modules that regulate protein interactions.},
  author={Yong Chen and Ren — Sheng and Morten K{\"a}llberg and Antonina Silkov and Moe Phyu Tun and Nitin Bhardwaj and Svetlana Kurilova and Randy A Hall and Barry Honig and Hui Lu and Wonhwa Cho},
  journal={Molecular cell},
  year={2012},
  volume={46 2},
  pages={226-37}
}
Emerging evidence indicates that membrane lipids regulate protein networking by directly interacting with protein-interaction domains (PIDs). As a pilot study to identify and functionally annodate lipid-binding PIDs on a genomic scale, we performed experimental and computational studies of PDZ domains. Characterization of 70 PDZ domains showed that ~40% had submicromolar membrane affinity. Using a computational model built from these data, we predicted the membrane-binding properties of 2,000… CONTINUE READING