Genome-wide detection and analysis of hippocampus core promoters using DeepCAGE.

  title={Genome-wide detection and analysis of hippocampus core promoters using DeepCAGE.},
  author={Eivind Valen and Giovanni Pascarella and A. E. C. L. Chalk and N. Maeda and Miki Kojima and Chika Kawazu and Mitsuyoshi Murata and Hiromi Nishiyori and Dejan Lazarevi{\'c} and Dario Motti and Troels Torben Marstrand and Man-Hung Eric Tang and Xiaobei Zhao and Anders Krogh and Ole Winther and Takahiro Arakawa and Jun Kawai and Christine A. Wells and Carsten O. Daub and Matthias Harbers and Yoshihide Hayashizaki and Stefano Gustincich and Albin Sandelin and Piero Carninci},
  journal={Genome research},
  volume={19 2},
Finding and characterizing mRNAs, their transcription start sites (TSS), and their associated promoters is a major focus in post-genome biology. Mammalian cells have at least 5-10 magnitudes more TSS than previously believed, and deeper sequencing is necessary to detect all active promoters in a given tissue. Here, we present a new method for high-throughput sequencing of 5' cDNA tags-DeepCAGE: merging the Cap Analysis of Gene Expression method with ultra-high-throughput sequence technology. We… CONTINUE READING
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