Genome-wide approaches to monitor pre-mRNA splicing.

Abstract

Pre-mRNA processing is an essential control-point in the gene expression pathway of eukaryotic organisms. The budding yeast Saccharomyces cerevisiae offers a powerful opportunity to examine the regulation of this pathway. In this chapter, we will describe methods that have been developed in our lab and others to examine pre-mRNA splicing from a genome-wide perspective in yeast. Our goal is to provide all of the necessary information--from microarray design to experimental setup to data analysis--to facilitate the widespread use of this technology.

DOI: 10.1016/S0076-6879(10)70003-3

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Cite this paper

@article{Inada2010GenomewideAT, title={Genome-wide approaches to monitor pre-mRNA splicing.}, author={Maki Inada and Jeffrey A. Pleiss}, journal={Methods in enzymology}, year={2010}, volume={470}, pages={51-75} }