Genome-wide approaches to determining nucleosome occupancy in metazoans using MNase-Seq.

@article{Cui2012GenomewideAT,
  title={Genome-wide approaches to determining nucleosome occupancy in metazoans using MNase-Seq.},
  author={Kairong Cui and Keji Zhao},
  journal={Methods in molecular biology},
  year={2012},
  volume={833},
  pages={413-9}
}
The precise location of nucleosomes in functional regulatory regions in chromatin is critical to the regulation of transcription. The nucleosome structure protects DNA from microccocal nuclease (MNase) digestion and leaves a footprint on DNA that indicates the position of nucleosomes. Short sequence reads (25-36 bp) from ends of mononucleosome-sized DNA generated from MNase digestion of chromatin can be determined using next-generation sequencing techniques. Mapping of these short reads to the… CONTINUE READING

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