Genome-wide analysis of the role of the antibiotic biosynthesis regulator AbsA2 in Streptomyces coelicolor A3(2)

@article{Lewis2019GenomewideAO,
  title={Genome-wide analysis of the role of the antibiotic biosynthesis regulator AbsA2 in Streptomyces coelicolor A3(2)},
  author={Richard A. Lewis and Abdul Wahab and Giselda Bucca and Emma E Laing and Carla S. M{\"o}ller-Levet and Andrzej M. Kierzek and Colin P. Smith},
  journal={PLoS ONE},
  year={2019},
  volume={14}
}
The AbsA1-AbsA2 two component signalling system of Streptomyces coelicolor has long been known to exert a powerful negative influence on the production of the antibiotics actinorhodin, undecylprodiginine and the Calcium-Dependent Antibiotic (CDA). Here we report the analysis of a ΔabsA2 deletion strain, which exhibits the classic precocious antibiotic hyper-production phenotype, and its complementation by an N-terminal triple-FLAG-tagged version of AbsA2. The complemented and non-complemented… Expand
3 Citations
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TLDR
The results establish that the abs genes affect production of antibiotics through regulation of expression of the antibiotic-specific regulatory genes in S. coelicolor through regulating transcript abundance from the biosynthetic and regulatory genes specific for each antibiotic. Expand
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TLDR
This paper has identified numerous transcriptional start sites within the CDA cluster and shown that the original antibiotic-negative mutants used to identify absA exhibit a stronger negative regulation of promoters upstream of the proposed CDA biosynthetic genes than of promoters in the clusters responsible for production of actinorhodin and undecylprodigiosin. Expand
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TLDR
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TLDR
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TLDR
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