Genome-wide analysis of the endothelial transcriptome under short-term chronic hypoxia.

@article{Ning2004GenomewideAO,
  title={Genome-wide analysis of the endothelial transcriptome under short-term chronic hypoxia.},
  author={Wei Ning and Tian Jiao Chu and Chiang J. Li and Augustine M. K. Choi and David G. Peters},
  journal={Physiological genomics},
  year={2004},
  volume={18 1},
  pages={70-8}
}
We have utilized serial analysis of gene expression (SAGE) to analyze the temporal response of human aortic endothelial cells (HAECs) to short-term chronic hypoxia at the level of transcription. Primary cultures of HAECs were exposed to 1% O2 hypoxia for 8 and 24 h and compared with identical same passage cells cultured under standard (5% CO2-95% air) conditions. A total of 121,446 tags representing 37,096 unique tags were sequenced and genes whose expression levels were modulated by hypoxia… CONTINUE READING