Genome-wide analysis of poly(A) site selection in Schizosaccharomyces pombe.

@article{Schlackow2013GenomewideAO,
  title={Genome-wide analysis of poly(A) site selection in Schizosaccharomyces pombe.},
  author={Margarita Schlackow and Samuel Marguerat and Nicholas Proudfoot and J{\"u}rg B{\"a}hler and Radek Erban and Monika Gullerova},
  journal={RNA},
  year={2013},
  volume={19 12},
  pages={1617-31}
}
Polyadenylation of pre-mRNAs, a critical step in eukaryotic gene expression, is mediated by cis elements collectively called the polyadenylation signal. Genome-wide analysis of such polyadenylation signals was missing in fission yeast, even though it is an important model organism. We demonstrate that the canonical AATAAA motif is the most frequent and functional polyadenylation signal in Schizosaccharomyces pombe. Using analysis of RNA-Seq data sets from cells grown under various physiological… CONTINUE READING