Genome-wide analysis of DNA copy-number changes using cDNA microarrays

@article{Pollack1999GenomewideAO,
  title={Genome-wide analysis of DNA copy-number changes using cDNA microarrays},
  author={Jonathan R. Pollack and Charles M. Perou and Ash A. Alizadeh and Michael B. Eisen and Alexander Pergamenschikov and Cheryl F. Williams and Stefanie S. Jeffrey and David Botstein and Patrick O. Brown},
  journal={Nature Genetics},
  year={1999},
  volume={23},
  pages={41-46}
}
Gene amplifications and deletions frequently contribute to tumorigenesis. Characterization of these DNA copy-number changes is important for both the basic understanding of cancer and its diagnosis. Comparative genomic hybridization (CGH) was developed to survey DNA copy-number variations across a whole genome. With CGH, differentially labelled test and reference genomic DNAs are co-hybridized to normal metaphase chromosomes, and fluorescence ratios along the length of chromosomes provide a… Expand
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