Genome-wide SWAp-tag yeast libraries for proteome exploration

@inproceedings{Weill2018GenomewideSY,
  title={Genome-wide SWAp-tag yeast libraries for proteome exploration},
  author={Uri Weill and Ido Yofe and Ehud Sass and Bram Stynen and Dan Davidi and Janani Natarajan and Reut Ben-Menachem and Zohar Avihou and Omer Goldman and Nofar Harpaz and Silvia Gabriela Chuartzman and Kiril Kniazev and Barbara Knoblach and Janina Laborenz and Felix Boos and Jacqueline Kowarzyk and Shifra Ben-Dor and Einat Zalckvar and Johannes M Herrmann and Richard A Rachubinski and Ophry Pines and Doron Rapaport and Stephen W. Michnick and Emmanuel D. Levy and Maya Schuldiner},
  booktitle={Nature Methods},
  year={2018}
}
  • Uri Weill, Ido Yofe, +22 authors Maya Schuldiner
  • Published in Nature Methods 2018
  • Medicine, Biology
  • Yeast libraries revolutionized the systematic study of cell biology. To extensively increase the number of such libraries, we used our previously devised SWAp-Tag (SWAT) approach to construct a genome-wide library of ~5,500 strains carrying the SWAT NOP1promoter-GFP module at the N terminus of proteins. In addition, we created six diverse libraries that restored the native regulation, created an overexpression library with a Cherry tag, or enabled protein complementation assays from two… CONTINUE READING

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