Genome-wide RNAi screening identifies human proteins with a regulatory function in the early secretory pathway

@article{Simpson2012GenomewideRS,
  title={Genome-wide RNAi screening identifies human proteins with a regulatory function in the early secretory pathway},
  author={Jeremy C Simpson and Brigitte Joggerst and Vibor Laketa and F{\'a}tima Ver{\'i}ssimo and Cihan Çetin and Holger Erfle and Mariana G. Bexiga and Vasanth R. Singan and Jean-Karim H{\'e}rich{\'e} and Beate Neumann and Alvaro Mateos and Jonathon Blake and Stephanie Bechtel and Vladimir Benes and Stefan Wiemann and Jan Ellenberg and Rainer Pepperkok},
  journal={Nature Cell Biology},
  year={2012},
  volume={14},
  pages={764-774}
}
The secretory pathway in mammalian cells has evolved to facilitate the transfer of cargo molecules to internal and cell surface membranes. Use of automated microscopy-based genome-wide RNA interference screens in cultured human cells allowed us to identify 554 proteins influencing secretion. Cloning, fluorescent-tagging and subcellular localization analysis of 179 of these proteins revealed that more than two-thirds localize to either the cytoplasm or membranes of the secretory and endocytic… CONTINUE READING
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