Genome-wide RNAi analysis of Caenorhabditis elegans fat regulatory genes

@article{Ashrafi2003GenomewideRA,
  title={Genome-wide RNAi analysis of Caenorhabditis elegans fat regulatory genes},
  author={Kaveh Ashrafi and Francesca Y. Chang and Jennifer L Watts and Andrew G. Fraser and Ravi S. Kamath and Julie Ahringer and Gary Ruvkun},
  journal={Nature},
  year={2003},
  volume={421},
  pages={268-272}
}
Regulation of body fat storage involves signalling between centres that regulate feeding in the brain and sites of fat storage and use in the body. Here we describe an assay for analysing fat storage and mobilization in living Caenorhabditis elegans. By using RNA-mediated interference (RNAi) to disrupt the expression of each of the 16,757 worm genes, we have systematically screened the C. elegans genome for genes necessary for normal fat storage. We identify 305 gene inactivations that cause… CONTINUE READING
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