Genome-wide LORE1 retrotransposon mutagenesis and high-throughput insertion detection in Lotus japonicus.

@article{Urbaski2012GenomewideLR,
  title={Genome-wide LORE1 retrotransposon mutagenesis and high-throughput insertion detection in Lotus japonicus.},
  author={Dorian Fabian Urbański and Anna Małolepszy and Jens Stougaard and Stig Uggerh\oj Andersen},
  journal={The Plant journal : for cell and molecular biology},
  year={2012},
  volume={69 4},
  pages={731-41}
}
Use of insertion mutants facilitates functional analysis of genes, but it has been difficult to identify a suitable mutagen and to establish large populations for reverse genetics in most plant species. The main challenge is developing efficient high-throughput procedures for both mutagenesis and identification of insertion sites. To date, only floral-dip T-DNA transformation of Arabidopsis has produced independent germinal insertions, thereby allowing generation of mutant populations from… CONTINUE READING

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