Genome shuffling of Lactobacillus fermentum for improved production of lactic acid

Abstract

The aim of this study was to investigate the effect of genome shuffling of Lactobacillus fermentum for improved production of lactic acid. Lactobacillus fermentum was mutagenized using nitrosoguanidine (NTG) while genome shuffling was carried out using standard method. Results showed that, only four strains (LB1-A, LB-17, LB-21 and LB-24) of the twenty nine strains mutagenized showed improved production of lactic acid as follows 26mg/L, 24mg/L, 40mg/L and 61mg/L as compared with the wild type (15mg/L). Also, after genome shuffling, some strains with improved lactic acid production were selected as follows; LB1-U, LB1-F, LB1-G and LB1-Z from the first experiment and LB-2D, LB – 2F, LB – 2J and LB – 2K from the second experiment. In conclusion, genome shuffling can be seen as an important tool for improving the production of lactic acid.

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Cite this paper

@inproceedings{Thomas2014GenomeSO, title={Genome shuffling of Lactobacillus fermentum for improved production of lactic acid}, author={Barry T. Thomas and G . C . Agu and S . O Makanjuola and O . D . Popoola}, year={2014} }