Intersection of genomics and health informatics approaches in identification of diseases’ biomarkers
- MedicineJournal of King Saud University - Science
A clinically annotated post-mortem approach to study multi-organ somatic mutational clonality in normal tissues
- Biology, MedicineScientific reports
The whole-body donor-based approach provides a nearly unlimited healthy tissue resource to study mutational clonality and gain fundamental mutagenic insights in the presumed earliest stages of tumor evolution.
A clinically annotated post-mortem approach to study multi-organ somatic mutational clonality in histologically healthy tissues
- Biology, MedicinebioRxiv
The whole-body donor-based approach provides a nearly unlimited healthy tissue resource to study mutational clonality and gain fundamental mutagenic insights in the presumed earliest stages of tumour evolution.
Spatiotemporal transcriptome at single-cell resolution reveals key radial glial cell population in axolotl telencephalon development and regeneration
This work systematically decoded the complex cellular and molecular dynamics of axolotl telencephalon in development and regeneration, laying the foundation for studying the regulatory mechanism of brain regeneration in the future.
Next Generation Sequencing: Principle and Applications
- Computer Science
High-Throughput Sequencing Technologies
- BiologyBioinformatics in Rice Research
This chapter presents a simple overview of the high-throughput sequencing technologies, their applications, and limitations to provide readers in the field with an easy and comprehensible description of HTS technologies to provide them with essential knowledge in full zeal.
Comparative Performance of the MGISEQ-2000 and Illumina X-Ten Sequencing Platforms for Paleogenomics
- BiologyFrontiers in Genetics
The results suggest that MGISEQ-2000 and X-Ten have comparable performance, and MGISEZ-2000 can be an alternative platform for paleogenomics sequencing.
Daily temperature cycles promote alternative splicing of RNAs encoding SR45a, a splicing regulator in maize
- BiologyPlant physiology
An “in protoplast” RNA splicing assay was established to show that during the afternoon on simulated hot summer days, SR45a RNA isoforms were produced with the potential to encode proteins efficient in splicing model substrates, a member of the SR45 gene family.
From gene to genomics
Advantages and Limitations of 16S rRNA Next-Generation Sequencing for Pathogen Identification in the Diagnostic Microbiology Laboratory: Perspectives from a Middle-Income Country
The basis for CBtest and 16SNGS is described, the limitations, challenges, advantages and future potential of using 16S NGS for bacterial pathogen identification in diagnostic microbiology laboratories of middle-income countries are discussed.
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Accurate Whole Human Genome Sequencing using Reversible Terminator Chemistry
An approach that generates several billion bases of accurate nucleotide sequence per experiment at low cost is reported, effective for accurate, rapid and economical whole-genome re-sequencing and many other biomedical applications.
The challenges of sequencing by synthesis
- BiologyNature Biotechnology
This work frames the challenges of DNA sequencing-by-synthesis in a manner accessible to a broad community of scientists and engineers, and hopes to solicit input from the broader research community on means of accelerating the advancement of genome sequencing technology.
Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome
We describe a DNA sequencing technology in which a commonly available, inexpensive epifluorescence microscope is converted to rapid nonelectrophoretic DNA sequencing automation. We apply this…
Sequence and structural variation in a human genome uncovered by short-read, massively parallel ligation sequencing using two-base encoding.
- BiologyGenome research
Dozens of mutations previously described in OMIM and hundreds of nonsynonymous single-nucleotide and structural variants in genes previously implicated in disease are identified in this individual.