Genome sequencing on nanoballs

  title={Genome sequencing on nanoballs},
  author={Gregory Porreca},
  journal={Nature Biotechnology},
  • G. Porreca
  • Published 1 January 2010
  • Biology, Medicine
  • Nature Biotechnology
Advances in technology deliver cheaper human genome sequencing. 
Comparative Performance of the MGISEQ-2000 and Illumina X-Ten Sequencing Platforms for Paleogenomics
The results suggest that MGISEQ-2000 and X-Ten have comparable performance, and MGISEZ-2000 can be an alternative platform for paleogenomics sequencing.
Daily temperature cycles promote alternative splicing of RNAs encoding SR45a, a splicing regulator in maize
An “in protoplast” RNA splicing assay was established to show that during the afternoon on simulated hot summer days, SR45a RNA isoforms were produced with the potential to encode proteins efficient in splicing model substrates, a member of the SR45 gene family.
High-Throughput Sequencing Technologies
  • E. Elumalai, Krishna Kant Gupta
  • Computer Science
    Bioinformatics in Rice Research
  • 2021
This chapter presents a simple overview of the high-throughput sequencing technologies, their applications, and limitations to provide readers in the field with an easy and comprehensible description of HTS technologies to provide them with essential knowledge in full zeal.
Spatiotemporal transcriptome at single-cell resolution reveals key radial glial cell population in axolotl telencephalon development and regeneration
This work systematically decoded the complex cellular and molecular dynamics of axolotl telencephalon in development and regeneration, laying the foundation for studying the regulatory mechanism of brain regeneration in the future.
Advantages and Limitations of 16S rRNA Next-Generation Sequencing for Pathogen Identification in the Diagnostic Microbiology Laboratory: Perspectives from a Middle-Income Country
The basis for CBtest and 16SNGS is described, the limitations, challenges, advantages and future potential of using 16S NGS for bacterial pathogen identification in diagnostic microbiology laboratories of middle-income countries are discussed.
The Transcription Factor bZIP60 Links the Unfolded Protein Response to the Heat Stress Response in Maize[OPEN]
A mutant knocking down bZIP60 expression blunted the HSR at elevated temperatures and prevented the normal upregulation of a group of heat shock protein genes in response to elevated temperature, quite unexpectedly contributes to the nuclear/cytoplasmic HSR in maize.
Clinical and Molecular Diagnosis in Muscular Dystrophies
This chapter presents the most important clinical and laboratory findings in the most common forms of muscular dystrophies and molecular diagnostic approaches for a more accurate diagnosis.


Sequence and structural variation in a human genome uncovered by short-read, massively parallel ligation sequencing using two-base encoding.
Dozens of mutations previously described in OMIM and hundreds of nonsynonymous single-nucleotide and structural variants in genes previously implicated in disease are identified in this individual.
The challenges of sequencing by synthesis
This work frames the challenges of DNA sequencing-by-synthesis in a manner accessible to a broad community of scientists and engineers, and hopes to solicit input from the broader research community on means of accelerating the advancement of genome sequencing technology.
Accurate Whole Human Genome Sequencing using Reversible Terminator Chemistry
An approach that generates several billion bases of accurate nucleotide sequence per experiment at low cost is reported, effective for accurate, rapid and economical whole-genome re-sequencing and many other biomedical applications.
Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome
We describe a DNA sequencing technology in which a commonly available, inexpensive epifluorescence microscope is converted to rapid nonelectrophoretic DNA sequencing automation. We apply this