Genome sequencing in microfabricated high-density picolitre reactors

  title={Genome sequencing in microfabricated high-density picolitre reactors},
  author={M. Margulies and M. Egholm and William E. Altman and S. Attiya and J. Bader and Lisa A. Bemben and J. Berka and Michael S. Braverman and Y. Chen and Z. Chen and Scott B. Dewell and Lei Du and J. M. Fierro and X. Gomes and B. Godwin and Wen He and Scott Helgesen and Chun He Ho and G. Irzyk and Szilveszter C. Jando and Maria L. I. Alenquer and T. Jarvie and K. Jirage and J. Kim and J. R. Knight and Janna R. Lanza and J. Leamon and Steven M. Lefkowitz and M. Lei and J. Li and K. L. Lohman and H. Lu and V. Makhijani and Keith E. McDade and Michael P. McKenna and Eugene W. Myers and E. Nickerson and J. Nobile and Ramona Plant and B. Puc and M. Ronan and G. T. Roth and Gary J. Sarkis and J. F. Simons and J. W. Simpson and M. Srinivasan and K. Tartaro and A. Tomasz and K. Vogt and Greg A. Volkmer and S. H. Wang and Y. Wang and Michael P. Weiner and Pengguang Yu and R. Begley and J. M. Rothberg},
  • M. Margulies, M. Egholm, +53 authors J. M. Rothberg
  • Published 2005
  • Biology, Medicine
  • Nature
  • The proliferation of large-scale DNA-sequencing projects in recent years has driven a search for alternative methods to reduce time and cost. Here we describe a scalable, highly parallel sequencing system with raw throughput significantly greater than that of state-of-the-art capillary electrophoresis instruments. The apparatus uses a novel fibre-optic slide of individual wells and is able to sequence 25 million bases, at 99% or better accuracy, in one four-hour run. To achieve an approximately… CONTINUE READING
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