Genome sequencing in microfabricated high-density picolitre reactors

@article{Margulies2005GenomeSI,
  title={Genome sequencing in microfabricated high-density picolitre reactors},
  author={Marcel Margulies and Michael Wayland Egholm and William E. Altman and Said Attiya and Joel S. Bader and Lisa A. Bemben and Jan Berka and Michael S. Braverman and Yi-Ju Chen and Zhoutao Chen and Scott B. Dewell and Lei Du and Joseph Miguel Fierro and Xavier V. Gomes and Brian Christopher Godwin and Wen He and Scott Helgesen and Chun He Ho and Gerard P. Irzyk and Szilveszter C. Jando and Maria L. I. Alenquer and Thomas P. Jarvie and Kshama B. Jirage and Jong-Bum Kim and James R. Knight and Janna R. Lanza and John Harris Leamon and Steven M. Lefkowitz and Ming Lei and Jing Li and Kenton L. Lohman and Hong Lu and Vinod B. Makhijani and Keith E. McDade and Michael P. McKenna and Eugene W. Myers and Elizabeth Nickerson and John R. Nobile and Ramona N Plant and Bernard Peter Puc and Michael T. Ronan and George T. Roth and Gary J. Sarkis and Jan Fredrik Simons and John W. Simpson and Maithreyan Srinivasan and Karrie R. Tartaro and Alexander Tomasz and Kari A. Vogt and Greg A. Volkmer and Shally H. Wang and Yong Wang and M. P. Weiner and Pengguang Yu and Richard F. Begley and Jonathan Rothberg},
  journal={Nature},
  year={2005},
  volume={437},
  pages={376-380}
}
The proliferation of large-scale DNA-sequencing projects in recent years has driven a search for alternative methods to reduce time and cost. Here we describe a scalable, highly parallel sequencing system with raw throughput significantly greater than that of state-of-the-art capillary electrophoresis instruments. The apparatus uses a novel fibre-optic slide of individual wells and is able to sequence 25 million bases, at 99% or better accuracy, in one four-hour run. To achieve an approximately… 
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