Genome sequence of the bioplastic-producing “Knallgas” bacterium Ralstonia eutropha H16

@article{Pohlmann2006GenomeSO,
  title={Genome sequence of the bioplastic-producing “Knallgas” bacterium Ralstonia eutropha H16},
  author={Anne Pohlmann and Wolfgang Florian Fricke and Frank Reinecke and Bernhard Kusian and Heiko Liesegang and Rainer Cramm and Thomas Eitinger and Christian Ewering and Markus Pötter and Edward Schwartz and Axel W. Strittmatter and Ingo Voss and Gerhard Gottschalk and Alexander Steinb{\"u}chel and Bärbel Friedrich and Botho Bowien},
  journal={Nature Biotechnology},
  year={2006},
  volume={24},
  pages={1257-1262}
}
The H2-oxidizing lithoautotrophic bacterium Ralstonia eutropha H16 is a metabolically versatile organism capable of subsisting, in the absence of organic growth substrates, on H2 and CO2 as its sole sources of energy and carbon. R. eutropha H16 first attracted biotechnological interest nearly 50 years ago with the realization that the organism's ability to produce and store large amounts of poly[R-(–)-3-hydroxybutyrate] and other polyesters could be harnessed to make biodegradable plastics… Expand
An Efficient Transformation Method for the Bioplastic-Producing "Knallgas" Bacterium Ralstonia eutropha H16.
TLDR
The optimized electroporation protocol achieves a transformation efficiency of (3.86 ± 0.29) × 105  cfu µg-1 DNA, a 103 -fold improvement compared to a previously published value for the same plasmid. Expand
Engineering the heterotrophic carbon sources utilization range of Ralstonia eutropha H16 for applications in biotechnology
TLDR
The conversion of diverse substrates to polyhydroxyalkanoates by R. eutropha was steadily improved by optimization of cultivation conditions, mutagenesis and metabolic engineering. Expand
Ralstonia eutropha Strain H16 as Model Organism for PHA Metabolism and for Biotechnological Production of Technically Interesting Biopolymers
TLDR
The recently published genome sequence of strain H16 allows researchers to take a closer look at the genetic potential of this versatile bacterium R. eutropha. Expand
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R. eutropha strain H16 is qualified to become a production platform strain for a large spectrum of products because of its remarkable metabolically versatility and the available repertoire of genetic tools. Expand
Genome-scale reconstruction and in silico analysis of the Ralstonia eutropha H16 for polyhydroxyalkanoate synthesis, lithoautotrophic growth, and 2-methyl citric acid production
TLDR
The genome-scale metabolic model, RehMBEL1391, successfully represented metabolic characteristics of R. eutropha H16 at systems level and can be employed as an useful tool for understanding its metabolic capabilities, predicting its physiological consequences in response to various environmental and genetic changes, and developing strategies for systems metabolic engineering to improve its metabolic performance. Expand
A proteomic view of the facultatively chemolithoautotrophic lifestyle of Ralstonia eutropha H16
TLDR
The results reported here are in agreement with earlier physiological and enzymological studies indicating that R. eutropha H16 has a heterotrophic core metabolism onto which the functions of lithoautotrophy have been grafted. Expand
EXPANDING MOLECULAR TOOLS FOR THE METABOLIC ENGINEERING OF RALSTONIA EUTROPHA H16
TLDR
The development of key molecular tools crucial to improving the biosynthesis of malonyl-CoA - a precursor metabolite required for the biosynthetic of fatty acids and potentially several valuable bio-products in Ralstonia eutropha H16 are focused on. Expand
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Megaplasmids of Aerobic Hydrogenotrophic and Carboxidotrophic Bacteria
TLDR
In both cases there is an interdigitation of megaplasmid-based and chromosomally encoded functions, indicating that thesemegaplasmids are, although not strictly essential for viability, an integral part of the genome. Expand
Genomic View of Energy Metabolism in Ralstonia eutropha H16
  • R. Cramm
  • Medicine, Biology
  • Journal of Molecular Microbiology and Biotechnology
  • 2008
TLDR
Analysis of the complete genome sequence of strain H16 revealed genes for several isoenzymes, permit assignment of well-known physiological functions to previously unidentified genes, and suggest the presence of unknown components of energy metabolism. Expand
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References

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TLDR
The complete nucleotide sequence of pHG1 is determined and the genes for conjugative plasmid transfer predict that R. eutropha forms two types of pili, related to the type IV pili of pathogenic enterobacteria. Expand
The methylcitric acid pathway in Ralstonia eutropha: new genes identified involved in propionate metabolism.
TLDR
From the molecular, physiological and enzymic analysis of Ralstonia eutropha HF39 null-allele mutants it was concluded that in this bacterium propionic acid is metabolized via the methylcitric acid pathway. Expand
The methylcitric acid pathway in Ralstonia eutropha: new genes identified involved in propionate metabolism
TLDR
From the molecular, physiological and enzymic analysis of Ralstonia eutropha HF39 null-allele mutants it was concluded that in this bacterium propionic acid is metabolized via the methylcitric acid pathway. Expand
A Megaplasmid-Borne Anaerobic Ribonucleotide Reductase in Alcaligenes eutrophus H16
TLDR
Analysis of transcriptional and translational fusions indicate that NrdD and nrdG are cotranscribed and that the translation efficiency of nrdD is 40-fold higher than that of NrdG, indicating that the two proteins Nrd D and N thirdG are not synthesized at a stoichiometric level. Expand
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TLDR
Using pulsed field gel electrophoresis and megabase DNA techniques to investigate the basic genomic organization of Ralstonia eutropha H16, and to construct a physical map of its indigenous megaplasmid pHG1, showed that genes for anaerobic metabolism are located on all three genomic replicons. Expand
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TLDR
The findings suggest that MoeA of R. eutropha is differentially involved in the biosynthesis or incorporation of pterin cofactors of/into the various molybdo- and tungstoenzymes. Expand
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TLDR
The synthesis and characterization of microbial polythioesters (PTEs), a novel class of biopolymers of general technological relevance, are reported, which open up new avenues in the field of biomaterials. Expand
Genetics and control of CO2 assimilation in the chemoautotroph Ralstoniaeutropha
TLDR
It is conceivable that specific variants of cbb control systems have evolved to ensure their optimal integration into regulatory networks operating in the diverse autotrophs characterized by different metabolic capabilities. Expand
Identification of cfxR, an activator gene of autotrophic CO2 fixation in Alcaligenes eutrophus
A regulatory gene, cfxR, involved in the carbon dioxide assimilation of Alcaligenes eutrophus H16 has been characterized through the analysis of mutants. The function of cfxR is required for theExpand
Formate and Oxalate Metabolism in Alcaligenes eutrophus
Summary: Alcaligenes eutrophus strain H16 when grown on formate or oxalate as the sole source of carbon and energy had doubling times between 3.5 and 4.5 h. The respective molar growth yields (Y m)Expand
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