Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR–Cas9 library

@article{Zhu2016GenomescaleDS,
  title={Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR–Cas9 library},
  author={Shiyou Zhu and Wei Li and Jingze Liu and Chen-Hao Chen and Qi Liao and Ping Xu and Han Xu and Tengfei Xiao and Zhongzheng Cao and Jingyu Peng and Pengfei Yuan and Myles Brown and Xiaole Shirley Liu and Wensheng Wei},
  journal={Nature Biotechnology},
  year={2016},
  volume={34},
  pages={1279-1286}
}
CRISPR–Cas9 screens have been widely adopted to analyze coding-gene functions, but high-throughput screening of non-coding elements using this method is more challenging because indels caused by a single cut in non-coding regions are unlikely to produce a functional knockout. A high-throughput method to produce deletions of non-coding DNA is needed. We report a high-throughput genomic deletion strategy to screen for functional long non-coding RNAs (lncRNAs) that is based on a lentiviral paired… CONTINUE READING