Genome organization and phylogenetic relationship of Pineapple mealybug wilt associated virus-3 with family Closteroviridae members

@article{Sether2009GenomeOA,
  title={Genome organization and phylogenetic relationship of Pineapple mealybug wilt associated virus-3 with family Closteroviridae members},
  author={Diane M. Sether and Michael J. Melzer and Wayne B. Borth and John S. Hu},
  journal={Virus Genes},
  year={2009},
  volume={38},
  pages={414-420}
}
The nucleotide sequence of Pineapple mealybug wilt associated virus-3 (PMWaV-3) (Closteroviridae: Ampelovirus), spanning seven open reading frames (ORFs) and the untranslatable region of the 3′ end was determined. Based on the amino acid identities with orthologous ORFs of PMWaV-1 (54%–73%) and PMWaV-2 (13%–35%), we propose PMWaV-3 is a new species in the PMWaV complex. PMWaV-3 lacks an intergenic region between ORF1b and ORF2, encodes a relatively small, 28.8 kDa, coat protein, and lacks a… 
Further genomic characterization of pineapple mealybug wilt-associated viruses using high-throughput sequencing
TLDR
The further genomic characterization of several pineapple mealybug wilt-associated virus (PMWaV) members of the genus Ampelovirus, family Closteroviridae using high-throughput sequencing (HTS) indicates PMWaV-4 is a distinct strain of PM WaV-1 but not a distinct ampelovirus species.
Complete genomic sequence of a Pineapple mealybug wilt-associated virus-1 from Hainan Island, China
TLDR
This is the first report of the complete genome sequence of Pineapple mealybug wilt-associated virus-1 (PMWaV-1 HN, KJ872494) identified on pineapple in Hainan island, China and based on Maximum Likelihood method, all conserved trees of RdRp, HSP70h and CP proteins sequences revealed that PMWaVs together with other PMWav-1 isolates clearly separated into PMWaV1 group.
Mealybug Wilt of Pineapple and Associated Viruses
TLDR
More research is necessary to explore the confusing etiology of the MWP disease, and to shed light upon the symptom development, it is possible that further, uncharacterized PMWaVs may be present in symptomatic pineapple plants that test negative for PMWaV-2, explaining the inconsistency in symptom development.
First report of Pineapple mealybug wilt associated virus-3 infecting pineapple in Cuba
TLDR
Pineapple mealybug wilt of pineapple (MWP) infection has been correlated with 5 to 15% of ratoon crop yield reduction and losses of up to 30% of production due to premature or asynchronous fruit ripeness in Hawaii, and these two viruses have similar deleterious effects on either growth rate or pineapple fruit yield.
Biological, Serological, and Molecular Characterization of a Highly Divergent Strain of Grapevine leafroll-associated virus 4 Causing Grapevine Leafroll Disease.
TLDR
The complete genome sequence of a highly divergent strain of Grapevine leafroll-associated virus 4 (GLRaV-4) was determined using 454 pyrosequencing technology and molecular and serological data revealed that the AA42 accession from which GL RaV-7 was originally reported is in fact co-infected with GLRa V-4 Ob and GLRav-7, challenging the idea that GLRaVs-7 is a leaf roll-causing agent.
A putative new ampelovirus associated with grapevine leafroll disease
A putative new ampelovirus was detected in Vitis vinifera cv. Carnelian showing mild leafroll symptoms and molecularly characterized. The complete genome consisted of 13,625 nt and had a structure
Novel Ampeloviruses Infecting Cassava in Central Africa and the South-West Indian Ocean Islands
TLDR
This study identifies for the first time and characterize the genome organization of novel ampeloviruses infecting cassava plants in diverse geographical locations using three high-throughput sequencing protocols and provides a first analysis of the diversity of these agents and of the evolutionary forces acting on them.
Transmission characteristics of Pineapple mealybug wilt associated virus-2 by the grey pineapple mealybugs Dysmicoccus neobrevipes in Hawaii.
TLDR
Data show that PMWaV-2 is transmitted by the grey pineapple mealybug in a semi-persistent manner, and a potential management strategy based on these findings is discussed.
...
1
2
...

References

SHOWING 1-10 OF 38 REFERENCES
Complete nucleotide sequence and genome organization of pineapple mealybug wilt-associated virus-1
TLDR
The complete genome of PMWaV-1 was sequenced and found to be 13.1 kb in length, making it the smallest in the family and unusual for an ampelovirus.
The genetic diversity of ampeloviruses in Australian pineapples and their association with mealybug wilt disease
TLDR
Phylogenetic analyses of selected regions of these sequences indicated that Pineapple mealybug wilt-associated virus 5 is a distinct species and most closely related to PMWaV-1, which was the most commonly found of the four viruses and conversely PM WaV-5 was only occasionally found.
Nucleotide sequence, genome organization and phylogenetic analysis of pineapple mealybug wilt-associated virus-2.
TLDR
The genome of pineapple mealybug wilt-associated closterovirus-2 was cloned from double-stranded RNA isolated from diseased pineapple and its sequence determined, andylogenetic analysis revealed that within the family CLOSTEROVIRIDAE: the mealy bug-transmitted PMWaV-2 is more closely related to other mealy bugs than to those which are transmitted by aphids or whiteflies.
Nucleotide sequence and genome organization of grapevine leafroll-associated virus-2 are similar to beet yellows virus, the closterovirus type member.
TLDR
GLRaV-2 is the only closterovirus, so far, that matches the genome organization of the type member of the group, BYV, and thus can be unambiguously classified as a definitive members of the genus Closterov virus.
Differentiation, Distribution, and Elimination of Two Different Pineapple mealybug wilt-associated viruses Found in Pineapple.
TLDR
Tissue blot immunoassays with two different monoclonal antibodies specific to either PM WaV-1 or PMWaV-2 indicated that both closteroviruses are widely distributed throughout the pineapple-growing areas of the world.
Molecular characterization and detection of plum bark necrosis stem pitting-associated virus
TLDR
The complete RNA genome of plum bark necrosis stem pitting-associated virus (PBNSPaV) was cloned and sequenced and was determined to be 14, 214 nts long, representing the simplest genome organization in the genus Ampelovirus.
Beet yellows closterovirus: complete genome structure and identification of a leader papain-like thiol protease.
TLDR
The 348K protein of BYV is longer than the putative replicases of the most closely related viruses by about 1300 amino acids distributed between two unique regions, one at the N-terminus, and the other in the central portion.
Diversity and Mealybug Transmissibility of Ampeloviruses in Pineapple.
TLDR
Plants infected with PMWaV-3 that were continuously exposed to mealybugs did not develop symptoms of MWP in the absence of PMWaVs, and the potential deletion mutant spanning the N-terminal of the HSP70 region was obtained from a pineapple accession from Zaire maintained at the USDA-ARS National Clonal Germplasm Repository in Hawaii.
Nucleotide sequence and organization of eight 3' open reading frames of the citrus tristeza closterovirus genome.
TLDR
A specific four-gene module consisting of thehsp70 protein, the hsp90-related protein, a diverged copy of the CP, and the CP itself was found to be common in organization between CTV and beet yellows closterovirus, indicating that these viruses probably have evolved from a common ancestor.
Complete nucleotide sequence of a South African isolate of grapevine leafroll-associated virus 3 reveals a 5′UTR of 737 nucleotides
TLDR
The sequence of GLRaV-3, isolate GP18, has a 50UTR of 737 nt and the 30-terminus of GP18 was found to be similar to that of the NY-1 isolate, and the efficacy of RLM-RACE to determine the 50-termini of multiple ssRNA viruses from total RNA in a single reaction was investigated.
...
1
2
3
4
...