Genome engineering reveals large dispensable regions in Bacillus subtilis.

  title={Genome engineering reveals large dispensable regions in Bacillus subtilis.},
  author={Helga Westers and Ronald Dorenbos and Jan Maarten van Dijl and Jorrit Kabel and Tony Flanagan and Kevin M Devine and Florence Jude and S. Seror and Aaron C Beekman and Elise Darmon and Caroline C Eschevins and Anne W F de Jong and Sierd Bron and Oscar P. Kuipers and Alessandra M. Albertini and Haike Antelmann and Michael Hecker and Nicola Zamboni and Uwe Sauer and Claude Bruand and Dusko S. Ehrlich and Juan Alonso and M. Salas and Wim J Quax},
  journal={Molecular biology and evolution},
  volume={20 12},
Bacterial genomes contain 250 to 500 essential genes, as suggested by single gene disruptions and theoretical considerations. If this view is correct, the remaining nonessential genes of an organism, such as Bacillus subtilis, have been acquired during evolution in its perpetually changing ecological niches. Notably, approximately 47% of the approximately 4,100 genes of B. subtilis belong to paralogous gene families in which several members have overlapping functions. Thus, essential gene… CONTINUE READING


Publications citing this paper.
Showing 1-10 of 61 extracted citations

Emerging tools for synthetic genome design.

Molecules and cells • 2013
View 1 Excerpt
Highly Influenced

Toward minimal bacterial cells: evolution vs. design.

FEMS microbiology reviews • 2009
View 4 Excerpts
Highly Influenced


Publications referenced by this paper.
Showing 1-10 of 66 references

Engineering a reduced Escherichia coli genome.

Genome research • 2002
View 2 Excerpts
Highly Influenced

Essential Bacillus subtilis genes.

Proceedings of the National Academy of Sciences of the United States of America • 2003
View 2 Excerpts

A novel class of heat

Devine, J. M. van Dijl

Microarray analysis of the Bacillus subtilis K - state : genome - wide expression changes dependent on ComK

K. Buttner, C. Scharf, M. Hecker
Mol . Microbiol . • 2002

Similar Papers

Loading similar papers…