Genome editing with engineered zinc finger nucleases

@article{Urnov2010GenomeEW,
  title={Genome editing with engineered zinc finger nucleases},
  author={Fyodor D. Urnov and Edward J Rebar and Michael C. Holmes and H. Steve Zhang and Philip D. Gregory},
  journal={Nature Reviews Genetics},
  year={2010},
  volume={11},
  pages={636-646}
}
Reverse genetics in model organisms such as Drosophila melanogaster, Arabidopsis thaliana, zebrafish and rats, efficient genome engineering in human embryonic stem and induced pluripotent stem cells, targeted integration in crop plants, and HIV resistance in immune cells — this broad range of outcomes has resulted from the application of the same core technology: targeted genome cleavage by engineered, sequence-specific zinc finger nucleases followed by gene modification during subsequent… 

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References

SHOWING 1-10 OF 105 REFERENCES
Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases
TLDR
The use of engineered ZFNs to introduce heritable mutations into a genome obviates the need for embryonic stem cell lines and should be applicable to most animal species for which early-stage embryos are easily accessible.
Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases
TLDR
It is demonstrated that a single injection of DNA or messenger RNA encoding ZFNs into the one-cell rat embryo leads to a high frequency of animals carrying 25 to 100% disruption at the target locus.
Targeted genome editing in human cells with zinc finger nucleases constructed via modular assembly.
TLDR
This work synthesized and tested hundreds of ZFNs to target dozens of different sites in the human CCR5 gene-a co-receptor required for HIV infection-and found that many of these nucleases induced site-specific mutations in the C CR5 sequence.
Targeted mutagenesis using zinc-finger nucleases in Arabidopsis.
TLDR
Data indicate that ZFNs can form the basis of a highly efficient method for targeted mutagenesis of plant genes and demonstrate efficient transmission of the ZFN-induced mutations.
Efficient Gene Targeting in Drosophila With Zinc-Finger Nucleases
TLDR
In the absence of a homologous donor DNA, ZFN cleavage led to the recovery of new mutants at three loci—y, ry and bw—through nonhomologous end joining (NHEJ) after cleavage.
Heritable Targeted Gene Disruption in Zebrafish Using Designed Zinc Finger Nucleases
TLDR
The frequency and precision of gene-disruption events observed suggest that this approach should be applicable to any loci in zebrafish or in other organisms that allow mRNA delivery into the fertilized egg.
Gene editing in human stem cells using zinc finger nucleases and integrase-defective lentiviral vector delivery
TLDR
Using IDLV delivery and ZFNs targeting distinct loci, high levels of gene addition are observed in a panel of human cell lines, as well as human embryonic stem cells, allowing rapid, selection-free isolation of clonogenic cells with the desired genetic modification.
Generation of a triple‐gene knockout mammalian cell line using engineered zinc‐finger nucleases
TLDR
The development of zinc‐finger nucleases (ZFNs) targeted to cleave three independent genes with known null phenotypes are reported, demonstrating the utility of ZFNs in multi‐locus genome engineering.
Rapid Mutation of Endogenous Zebrafish Genes Using Zinc Finger Nucleases Made by Oligomerized Pool ENgineering (OPEN)
TLDR
This study nearly triples the total number of endogenous zebra fish genes successfully modified using ZFNs and suggests that OPEN provides a reliable method for introducing targeted mutations in nearly any zebrafish gene of interest.
High frequency targeted mutagenesis in Arabidopsis thaliana using zinc finger nucleases
TLDR
The high frequency of observed ZFN-induced mutagenesis suggests that targeted mutations can readily be recovered by simply screening progeny of primary transgenic plants by PCR and DNA sequencing.
...
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2
3
4
5
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