Genome editing in human stem cells.

@article{Byrne2014GenomeEI,
  title={Genome editing in human stem cells.},
  author={Susan M. Byrne and Prashant Mali and George M Church},
  journal={Methods in enzymology},
  year={2014},
  volume={546},
  pages={119-38}
}
The use of custom-engineered sequence-specific nucleases (including CRISPR/Cas9, ZFN, and TALEN) allows genetic changes in human cells to be easily made with much greater efficiency and precision than before. Engineered double-stranded DNA breaks can efficiently disrupt genes, or, with the right donor vector, engineer point mutations and gene insertions. However, a number of design considerations should be taken into account to ensure maximum gene targeting efficiency and specificity. This is… CONTINUE READING
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