Genome editing in Drosophila melanogaster: from basic genome engineering to the multipurpose CRISPR-Cas9 system

  title={Genome editing in Drosophila melanogaster: from basic genome engineering to the multipurpose CRISPR-Cas9 system},
  author={X. Ren and Kristof Holsteens and H. Li and J. Sun and Y. Zhang and L. Liu and Qingfei Liu and Jian-Quan Ni},
  journal={Science China Life Sciences},
  • X. Ren, Kristof Holsteens, +5 authors Jian-Quan Ni
  • Published 2017
  • Biology, Medicine
  • Science China Life Sciences
  • Nowadays, genome editing tools are indispensable for studying gene function in order to increase our knowledge of biochemical processes and disease mechanisms. The extensive availability of mutagenesis and transgenesis tools make Drosophila melanogaster an excellent model organism for geneticists. Early mutagenesis tools relied on chemical or physical methods, ethyl methane sulfonate (EMS) and X-rays respectively, to randomly alter DNA at a nucleotide or chromosomal level. Since the discovery… CONTINUE READING
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