Genome analysis of the moss Physcomitrella patens (Hedw.) B.S.G.

@article{Reski2004GenomeAO,
  title={Genome analysis of the moss Physcomitrella patens (Hedw.) B.S.G.},
  author={R. Reski and M. Faust and Xiao-Hui Wang and M. Wehe and W. Abel},
  journal={Molecular and General Genetics MGG},
  year={2004},
  volume={244},
  pages={352-359}
}
A wild-type (WT) strain of the moss Physcomitrella patens (Hedw.) B.S.G., two mutants derived from it (PC22 and P24), and a somatic hybrid, PC22(+)P24, were analysed. Staining of metaphases revealed 54±2 chromosomes in the somatic hybrid and 27 chromosomes in the wild type and the two mutants. Using flow cytometry (FCM), DNA contents were calculated to be 0.6 pg (WT, PC22), 1.2 pg (P24), and 1.6 pg (PC22(+)P24) per nucleus, respectively. Southern hybridization provided evidence for at least one… Expand
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