Genome-Wide Tuning of Protein Expression Levels to Rapidly Engineer Microbial Traits.


The reliable engineering of biological systems requires quantitative mapping of predictable and context-independent expression over a broad range of protein expression levels. However, current techniques for modifying expression levels are cumbersome and are not amenable to high-throughput approaches. Here we present major improvements to current techniques… (More)
DOI: 10.1021/acssynbio.5b00133

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