Genome-Wide RNAi Screens in C. elegans to Identify Genes Influencing Lifespan and Innate Immunity.

Abstract

RNA interference is a rapid, inexpensive, and highly effective tool used to inhibit gene function. In C. elegans, whole genome screens have been used to identify genes involved with numerous traits including aging and innate immunity. RNAi in C. elegans can be carried out via feeding, soaking, or injection. Here we outline protocols used to maintain, grow, and carry out RNAi via feeding in C. elegans and determine whether the inhibited genes are essential for lifespan or innate immunity.

DOI: 10.1007/978-1-4939-6337-9_14

Cite this paper

@article{Sinha2016GenomeWideRS, title={Genome-Wide RNAi Screens in C. elegans to Identify Genes Influencing Lifespan and Innate Immunity.}, author={Amit U. Sinha and R Rae}, journal={Methods in molecular biology}, year={2016}, volume={1470}, pages={171-82} }