Genome Walking by Next Generation Sequencing Approaches

  title={Genome Walking by Next Generation Sequencing Approaches},
  author={Mariateresa Volpicella and Claudia Leoni and Alessandra Costanza and Immacolata Fanizza and Antonio Placido and Luigi R. Ceci},
Genome Walking (GW) comprises a number of PCR-based methods for the identification of nucleotide sequences flanking known regions. The different methods have been used for several purposes: from de novo sequencing, useful for the identification of unknown regions, to the characterization of insertion sites for viruses and transposons. In the latter cases Genome Walking methods have been recently boosted by coupling to Next Generation Sequencing technologies. This review will focus on the… CONTINUE READING
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A Feasibility Study

  • Genotyping by Sequencing on Barley Using Ion PGMTM Sequencer
  • Available online:…
  • 2012
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