Genome Transplantation in Bacteria: Changing One Species to Another

@article{Lartigue2007GenomeTI,
  title={Genome Transplantation in Bacteria: Changing One Species to Another},
  author={C. Lartigue and J. Glass and Nina Alperovich and R. Pieper and P. Parmar and C. Hutchison and Hamilton O. Smith and J. Venter},
  journal={Science},
  year={2007},
  volume={317},
  pages={632 - 638}
}
As a step toward propagation of synthetic genomes, we completely replaced the genome of a bacterial cell with one from another species by transplanting a whole genome as naked DNA. Intact genomic DNA from Mycoplasma mycoides large colony (LC), virtually free of protein, was transplanted into Mycoplasma capricolum cells by polyethylene glycol–mediated transformation. Cells selected for tetracycline resistance, carried by the M. mycoides LC chromosome, contain the complete donor genome and are… Expand
Cloning and Transplantation of the Mesoplasma florum Genome.
Synthetic Genomics and the Construction of a Synthetic Bacterial Cell
  • J. Glass
  • Biology, Medicine
  • Perspectives in biology and medicine
  • 2012
Budding yeast as a factory to engineer partial and complete microbial genomes
Genomic content of Neisseria species.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 23 REFERENCES
Gene transfer in Mycoplasma pulmonis.
Combining two genomes in one cell: stable cloning of the Synechocystis PCC6803 genome in the Bacillus subtilis 168 genome.
Essential genes of a minimal bacterium.
Rebuilding microbial genomes.
Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector.
  • H. Shizuya, B. Birren, +4 authors M. Simon
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1992
Cell reproduction and morphological changes in Mycoplasma capricolum.
Studies on transformation of Escherichia coli with plasmids.
  • D. Hanahan
  • Biology, Medicine
  • Journal of molecular biology
  • 1983
...
1
2
3
...